Abstract:ObjectiveTo investigate the protective effect of baicalin on cerebral injury induced by transient focal ischemia and its mechanisms. MethodsTransient focal cerebral ischemia injury model in rats was induced by occlusion of the right middle cerebral artery for 2 h, followed by 24 h reperfusion. The infarction volume and neurological deficit determined by the methods of TTC staining and the Longa's score were used to evaluate the effect of baicalin on cerebral injury. The levels of malondialdehyde (MDA), nitric oxide (NO), activities of superoxide dismutase (SOD) and myeloperoxidase (MPO) and blood-brain barrier (BBB) permeability in brain were measured by spectrophotometer. The content of brain water was assayed by weighing method.ResultsPretreatment with baicalin markedly reduced brain infarction volume and neurological deficit induced by transient ischemic insult, inhibited MPO activity, decreased NO, MDA and brain water content, and BBB permeability, increased SOD activity.ConclusionBaicalin might play a protective effect on cerebral ischemia reperfusion injury through inhibiting inflammatory process, peroxidation and decreasing brain water contents.
Key words:Baicalin; Cerebral ischemia; Reperfusion injury; Brain water content
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