Abstract:ObjectiveTo establish a method of determining Podophllotoxin in Dysosma versipellis by HPLC.MethodsHigh Performance Liquid Chromatography was performed Diamonsil C18. The chromatography conditions were as follows: methanol - 0.1mol/L monobasic potassium phosphate (50∶50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.ResultsA good linearity of Podophllotoxin was shown in range of 0.047 69~0.476 9 mg/ml (r=0.999 6), the average recovery was 99.94% and RSD was 1.71%(n=5). ConclusionThe method can supply evidence for the quality evaluation of Dysosma versipellis. It is simple and accurate.