Abstract:ObjectiveTo research antimicrobial activity of Liquidambar formosana Hance's leaf.MethodsBy methods of agar plate diffusions and tube continuous dilution, to measure antimicrobial activity and minimal inhibitory concentration(MIC) of Liquidambar formosana Hance's leaf against pathogen and conditioned pathogen.Results(1) Liquidambar formosana Hance's leaf had not antimicrobial activity on E.coli, S.albicans. (2) Liquidambar formosana Hance's leaf had antimicrobial activity against S.aureus , S.epidermidis, S.flexneri, Salmonella Typhi, P.aeruginosa, the diameter of bacteriostatic circle was between 13 and 25 millimetre . The antimicrobial activity was significant different in different extracted drug liquid, among which the antimicrobial activity of water extraction of Liquidambar formosana Hance's leaf was the best,the minimal inhibitory concentration against S.aureus S.epidermidis, S.flexneri, Salmonella Typhi, P.aeruginosa, is 0.5,1,0.5,0.5,0.25 g/ml,respectively.ConclusionLiquidambar formosana Hance's leaf has good antimicrobial activity against S.aureus, S.epidermidis, S.flexneri, Salmonella Typhi and P.aeruginosa.