The Effects of Extracts from Ruixianglangdu and Tumour Necrosis Factor of Expression of Gene Protein in Two Lung Carcinoma Cell
Abstract:ObjectiveTo research the effect of extracts from Ruixianglangdu and tumour necrosis factor(TNF) on expression of gene protein .MethodsTwo lung carcinoma cell strains were treated with extracts from Ruixianglangdu and TNF,the expressions of MDM2,LRP and HSP27 in lung cancer cell strains were detected with immunohistochemistry SP assay before and after being treated respectively. To compare the difference of expression of three kinds of gene protein with morphological image assay.ResultsThe difference of expressive intensity of MDM2,LRP and HSP27 between before and after being treated with extracts from Ruixianglangdu and TNF in two lung carcinoma cell strains as follows. ①In Ruixianglangdu group:in lung carcinoma cell strain NCI-H446 the expressive intensity of LRP and MDM2 were higher than that before treated(P<0.05),except HSP 27(P>0.05).②In NCI-H157 the expressive intensity of LRP and HSP 27 were higher than that after treated(P<0.05),except MDM2 (P>0.05). ③In TNF group: the expressive intensity of LRP was higher than that before treated with TNF(P<0.05).The difference of expressive intensity of HSP27,MDM2 were inapparent before and after treated with TNF(P>0.05). ④In combination group: the restraining effect of expression of gene protein(MDM2,LRP and HSP27) were consuming in NCI-H446 and NCI-H157 strains(P<0.01). ConclusionThe extracts of Ruixianglangdu and TNF restrain the expression of certain kinds of gene protein in two lung carcinoma cell strains. The restraining effect of expression of gene protein are consuming in combination group.
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