Study of Angiogenesis and Correlative Growth Factors Effects of Salvia miltiorrhiza Injection on the Ischemic Myocardium of the Rats after Myocardial Infarction
Abstract:ObjectiveTo study the angiogenesic effects and mechanisms of Salvia miltiorrhiza Injection on the ischemic myocardium of the rats after myocardial infarction(MI).MethodsMale wistar rats were ligated left coronary artery to be made into models of acute myocardial infarction (AMI). All rats were randomly divided into Salvia miltiorrhiza Injection, Shexiang baoxin pills (positive control group), model (negative contral group) group and sham operation group. Treated them respectively for six weeks. Microvascular count(MVC), microvascular density (MVD), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), insulin-kind growth factor (IGF) and platelet-derived endothelial growth factor (PDGF) in ischemic myocardium of MI rats were detected by immunobistochemistry assay. The values of protein expression of VEGF,bFGF, PDGF-β and IGF-1 on ischemic myocardyzum were calculated and analied by image analysis system.Results There were more MVC and MVD in ischemic myocardium of AMI rats of model group、salvia miltiorrhiza injection group, Shexiang baoxin pills group than sham operation group. MVC and MVD were higher in ischemic myocardium of AMI rats of Salvia miltiorrhiza Injection group than those of model group. Compared with Shexiang baoxin pills, they had the same roll. The values of protein expression of VEGF, bFGF, PDGF-β and IGF-1 in model group were significantly elevated compared with that in sham operation group. The protein expression of VEGF, bFGF and PDGF-β in salvia miltionrrhiza injection group was significantly elevated compared with that in sham operation group. The protein expression of VEGF,bFGF and PDGF-β in Salvia miltiorrhiza Injection group was significantly elevated compared with that in model group. IGF- 1 in Salvia miltiorrhiza Injection group was lower than modle group. But there was no significant statistics difference between them. There were significant statistics differences in the protein expression of VEGF,bFGF,PDGF-βand IGF-1 in salvia miltiorrhiza injection group compared with that in Shexiang baoxin pills group. Conclusion Salvia miltiorrhiza Injection has the effect of promoting angiogenesis of the ischemic myocardium in rats after MI. Its mechanisms can be related with stimulating the expression of VEGF,bGFG and PDGF-β.
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