The Inducing Diarrhea Effects and its Mechanisms of Anthraquinon from Rheum palmatum L.
TANG Daxuan,TAN Zhenghuai,LIANG Yuanyuan,CHENG Lei,HUANG Li
1.Sichuan Institute of Chinese Materia Medica, Chengdu 610041,China;
2.Yunnan Dali College, Yunnan 671000,China
Abstract:ObjectiveTo compare the efficiency and mechanisms of diarrhea induced by anthraquinon and glycosides of anthraquinone from Rheum Palmatum L. in mice. MethodsThe lapactic effects of anthraquinon and glycosides of anthraquinone from Rheum Palmatum L. were studied in the mice which were given high concentration of powdered milk solution to induced supernutrition, and the effects of these drugs on the activity of pepsin were measured in vitro. The effect of milk on the lapactic action of anthraquinon and glycosides of anthraquinone were analyzed using the factorial design. ResultsThe anthraquinone of Rheum palmatum L. 500,1 000 mg/kg significantly increased the remnant in the gastric and small intestine in mice, and the glycosides of anthraquinone from Rheum Palmatum L. 500,1 000 mg/kg also significantly increased remnant in small intestine in mice. All these drugs could increase the concentration of protein in the gut in mice. The results of the factorical design showed that the anthraquinone or glycosides of anthraquinone of Rheum palmatum L. were able to remarkably increase the remnant and protein concentration in stomach, small intestine and all of gastrointestinal tract, reduce the concentration of Cl- in small intestine and colon, increase the concentration of K+ in colon in normal mice. Milk had the similar effect and could enhance the action of anthraquinone of Rheum palmatum L. In vitro, anthraquinone or glycosides of anthraquinone from Rheum palmatum L. had no action on the activity of pepsin. ConclusionThese results indicated that antharquinone or glycosides of anthraquinone from Rheum palmatum L. can induce diarrhea in mice. These actions may due to that anthraquinone can increase the secretion of protein in gut.
Key words:Anthraquinone from Rheum Palmatum L.(ARP); Glycosides of anthraquinone from Rheum Palmatum L .(GARP); Stomach; Small intestine; Colon
1.1 药物大黄结合蒽醌(Glycosides of Anthraquinone from Rheum palmatum L.,以下简称GARP):大黄经水煮提取后再用大孔树脂进行分离纯化,其大黄蒽醌主要为结合型,其纯度为36.5%;大黄游离蒽醌(Anthraquinone from Rheum palmatum L.,以下简称ARP), 其游离蒽醌含量为85.6%药, 以上实验用药均由四川省中药研究所药化室易进海博士提供。临用前用蒸馏水或40%奶粉溶液配制成所需浓度的混悬液备用。通便灵胶囊,0.25 g/粒,通化白山制药五厂生产,批号20020601-0249,临用前用蒸馏水配成所需浓度备用。伊利全脂甜奶粉,内蒙古伊利实业集团股份有限公司,批号8712880。临用前用蒸馏水配成所需浓度备用。