Study of the UV Absorption Spectroscopy and Fluorescence Spectroscopy of the Herbal Medicine Soldago decurrens Lour
Abstract:ObjectiveTo determine the chemical spectral character of Soldago decurrens Lour.MethodsSpectroscopic analysis of Soldago decurrens Lour was performed with UV absorption spectroscopy and fluorescence spectroscopy. ResultsUltraviolet spectral analysis show that the absorption of Soldago decurrens Lour were at 665, 606, 535 and 504 nm respectively. It exhibited one intense fluorescent emission band at 674 nm. ConclusionThe methods of UV absorption spectra and Fluorescence spectra can identify Soldago decurrens Lour swiftly and accurately. The methods are easy and simple to operate, and it also has high sensitivity and good repetition. In addition, the diagram that we have got has the characteristic of fingerprint.
1.1 材料样品溶液的制备:200506上旬一枝黄芪采摘于万载县慈化镇的山坡,经宜春学院医学院邓宽富副教授鉴定;一枝黄花用自来水洗净泥砂等杂质,然后用去离子水冲洗3~5次,常温下凉干,备用。取一枝黄花全草(干)30.0 g用100 ml 95%乙醇溶液浸泡7 d,并超声振荡,收集滤液;在滤渣中再加入150 ml 95%乙醇溶液浸泡7 d,收集滤液;再在滤渣中加入150 ml 95%乙醇溶液浸泡7 d,收集滤液。合并3次滤液,混匀作为供试液(75 mg/ml)。