Abstract:ObjectiveTo investigate the immunologic enhancement of Dioscorea opposita Thunb.polysaccharide.MethodsDioscorea opposita Thunb.polysaccharide in different dosages(200,400,800 mg/kg)were given by IG for 8 d.A series of tests were carried out,such as the spleen lymph cells transformation of Con A inducing method (MTT method),hemolytic test of blood serum and hem chrome and mouse carbon test.ResultsThe results showed that Dioscorea opposita Thunb.polysaccharide was significant to Con A induced mice Lymphocyte transformation test;HC50 raised in all three concentrations;ratio of monocyte-macrophage carbon grain elimination has significant changes to the control.ConclusionDioscored opposita Thunb.polysaccharide possesses a potential effect of immune regulation.