Analysis and Determination of Flavonoids in Pteris semipinnata L. by HPLC
Abstract:ObjectiveTo analyze and determine the content of luteolin and apigenin in Pteris semipinnata L. by HPLC. MethodsThe HPLC system consisted of ODS-C18 column(25 mm×4.6 mm, 5 μm), methanol : 0.4% sodium mixture as a mobile phase, detection wavelength at 345nm, 1 ml·min-1 of flow rate and column temperature at 25℃.ResultsThe linear range of luteolin and apigenin were 0.02~0.4 μg,r=0.999 9 and 0.02~0.4 μg,r=0.999 6 respectively,the recovery were 96.39%(RSD=1.95%,n=5) and 98.10%(RSD=0.96%,n=5)respectively.ConclusionThe method is simple, accurate, sensitive and reproducible. This method can be used for the quantitative determination of flavonoids in Pteris semipinnata L.