Abstract:ObjectiveTo establish the method for the quantitative determination of total flavonoids in Herba Leonuri, make the prime examination of the resources.MethodsThe sample was treated with a polyamide column for separation and purification ,and was colorated with aluminium trichloride ,and then,the content of flavone in the sample was determined at the wavelength of 412 nm by ultraviolet spectrophotometer with rutin as control.ResultsIt showed a good linear relationship in the range of 4.8~29.0 μg/ml,r=0.9996 the average recovery of the added sample was 102% and RSD was 3% .ConclusionThe method is simple and accurate. It can be used for the quality control.
Key words:Herba Leonuri; Total flavonoids; Column chromatogram UV spectrophotometry
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