Abstract:ObjectiveTo investigate the constituents of Pyrola xinjiangensis.. MethodsSeparation and purification were performed on silica gel CC and sephadex LH-20. Their structures were established on the basis of physicochemical and spectral analysis. ResultsFive compounds were isolated and identified as Pyrolin(Ⅰ), Isoquercitrin(Ⅱ), Pirolatin(Ⅲ), Monotropein(Ⅳ), renifolin(Ⅴ), respectively. ConclusionThese compounds are isolated from Pyrola xinjiangensis for the first time.
Key words:Pyrola xinjiangensis Y. L. Chou; Chemical constituents
新疆鹿蹄草Pyrola xinjiangensis Y. L. Chou为鹿蹄草科鹿蹄草属植物,产于新疆维吾尔自治区境内的天山及阿尔泰山脉,是新疆民族药常用药材[1]。该属植物共有三十余种,我国产27种3变种,较为集中的分布在我国的西南部和东北部[2]。《中国药典》中收载的中药鹿蹄草是鹿蹄草或普通鹿蹄草的干燥全草,其性温,味甘、苦,具有祛风除湿、强壮筋骨、补虚益肾、收敛止血的功效,主治风湿痹痛,肾虚盗汗,筋骨酸软,虚弱咳嗽,外伤出血。哈萨克民间用新疆鹿蹄草治疗和预防心血管疾病,对冠心病、高血压病以及由其引发的心痛、胸闷、心悸等有特效。体外抗血小板聚集活性测试表明新疆鹿蹄草的70%乙醇提取物对血小板聚集有显著的抑制作用。本实验对新疆鹿蹄草乙醇提取物的正丁醇萃取部分进行了化学成分分离,从中得到五个化合物,通过化学和光谱方法鉴定了它们的结构,分别为鹿蹄草素(Ⅰ),异槲皮苷(Ⅱ),鹿蹄草苷(Ⅲ),水晶兰苷(Ⅳ),肾叶鹿蹄草苷(Ⅴ),所有化合物均为首次从新疆鹿蹄草中获得。
1 仪器与材料
Yanaco显微熔点测定仪(温度未校正),FTS165型红外光谱仪(美国PerkinElmer公司生产),EI-MS和FABMS用ZABHS型质谱仪,Varian inova400型核磁共振仪(TMS作内标)。柱色谱硅胶(200300目)和薄层色谱硅胶GF254均为青岛海洋化工厂产品,sephadex LH20为Pharmacia公司产品。化学试剂均为分析纯。药材购自新疆阿勒泰,由新疆生态与地理研究所沈观冕研究员鉴定为新疆鹿蹄草Pyrola xinjiangensis Y. L. Chou。
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