Determination of Puerarin Content in Jiangtang Pill for Invigorating Qi Nourishing Yin by HPLC Method
Abstract:ObjectiveTo develop a method to determine the content of puerarin in Jiangtang pill for invigorating Qi, nourishing Yin.MethodsHigh Performance Liquid Chromatography (HPLC), with detection wavelength of 250 nm, the mobile phase consists of methanol and 0.1% citric acid (25∶75), at flow rate∶1.0 ml/min.ResultsThe linear range of puerarin was 10.0~90.0 μg/ml(r=0.999 6). The recovery of sample was 99.5%(n=3) with RSD of 1.2%.ConclusionThis method is simple accurate, and can be used for the determination of puerarin content in Jiangtang pill for invigorating Qi, nourishing Yin.
Key words:HPLC method; Puerarin; Jiangtang pill for invigorating Qi