【摘要】 目的建立复方银杏注射液中银杏总黄酮醇苷的含量测定方法。方法高效液相色谱法, Zorbax-Extend-C18柱(5 μm ,4.6 mm ×150 mm );甲醇0.4%磷酸溶液(45∶55)为流动相,流速为1.0 ml·min-1,柱温30℃,检测波长360 nm。结果槲皮素浓度在2.07~69.00 μg·ml-1,山柰素浓度在1.91~63.60 μg·ml-1,异鼠李素浓度在0.73~24.20 μg·ml-1范围内呈良好线性关系。结论该方法分析时间短、专属性好、稳定,可以作为复方银杏注射液质量控制方法。
【关键词】 复方银杏注射液; 总黄酮; 高效液相色谱法
Abstract:ObjectiveTo establish a method for determining the flavonoids in Compound Ginkgo biloba Injection by HPLC. MethodsHPLC, Zorbax-Extend-C18 column (5 μm ,4. 6 mm×150 mm) was used. The mobile phase consisted of methanol0.4% phosphoric acid (45∶55)with flow rate of 1.0 ml·min-1.The detection wavelength was set at 360 nm .The column temperature was set at 30℃ .ResultsThe linear range of quercetin, kaempferol and isorhamnetin were at the range of 2.07~69.00 μg·ml-1, 1.91~63.60 μg·ml-1 and 0.73~24.20 μg·ml-1. ConclusionThe method is rapid ,exclusive and steady, and can be used for the quality control for the injection.