Abstract:ObjectiveTo optimize refinement of Carthamus tinctorius L. by chitosan flocculation.MethodsApplying the L9(34)of orthogonal design and high performance liquid chromatography(HPLC), with the yield rate of hydroxy safflower yellow A. as the index. ResultsThe optimized condition was A3B2C1, which was that medicinal herb: physic liquor=1:6, the addition of chitosan was 0.4 ml/g medicinal herb, setting 6 hours.ConclusionChitosan flocculation method was effective and economic for the clarification and can be used in the refinement of Carthamus tinctorius L.