Abstract:ObjectiveTo establish the quality standard for Wumiao Shuixian Cream.MethodsCortex Phellodendri chinense,Radix arnebiae and Galla chinesis in this prescription were identified by TLC.The content of the berberine was determined by HPLC: the column of AichromBondAQC18(4.6 mm×150 mm,5 μm)was used . The mobile phase was CH3CN-0.033 mol/LKH2PO4(trithylamine adjusted pH to 3.0)-0.025 mol/L SDS (48∶30∶25),at the flow rate of 1.0 ml·min-1,the column temperature was 30℃,and detection wavelength was 345 nm.ResultsThe study on the quality control showed that the characteristic of identification by TLC was distinct and highly specific.The linear range of berberine was in the range of 0.07~0.28μg,r= 0.9995,the average recovery was 98.11%,RSD=1.34%(n=6).ConclusionThe method is simple,accurate and reproducible.It can be used effectively for the quality control of this preparation.