人恶性胶质瘤细胞系U87MG由南方医科大学神经生物学教研室保存,A431细胞购自中山大学实验动物中心细胞库。pcDNA4/TOEGFRvIII质粒由University of Texas Southwestern Medical Center的Amyn A. Habib教授惠赠。DMEM培养基及Zeocin购自Invitrogen公司,胎牛血清购自杭州四季青公司,小鼠抗EGFR单克隆抗体购自Biosource公司,RNAiso试剂购自TaKaRa公司,RTPCR试剂盒购自Promega公司,RTPCR引物在TaKaRa公司合成。
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